Read more at: New paper out from Jewett lab on cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates
New paper out from Jewett lab on cell-free biosynthesis of limonene using enzyme-enriched Escherichia coli lysates
6 March 2019
Isoprenoids are an attractive class of metabolites for enzymatic synthesis from renewable substrates. However, metabolic engineering of microorganisms for monoterpenoid production is limited by the need for time-consuming, and often non-intuitive, combinatorial tuning of biosynthetic pathway variations to meet design criteria. Towards alleviating this limitation, the goal of this work was to build a modular, cell-free platform for construction and testing of monoterpenoid pathways, using the fragrance and flavoring molecule limonene as a model.